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        06021-58830-11
        Mod:06021-58830-11
        Fax:06021-58830-0
        Add:Zeppelinstr. 5, 63741 Aschaffenburg, Germany

        HPLC Columns

        Target carbohydrate analysis column


                           
                          (packing resin and structure diagram of target carbohydrate analysis chromatography)


        Target carbohydrate series analytical column is composed of polystyrene /  (PS / DVB) as the matrix, cross linked with various ions. Taget There are three crosslinking degrees (5%, 8%, 10%) and five kinds of ions, such as H +, Ca2 +, Pb2 +, K +, Na +, are found in the packing of carbohydrate series analytical column; It is widely used in the separation and preparation of organic acids and carbohydrates, polypeptides and small biomolecules in cell metabolites.

        Characteristics of target carbohydrate analysis column
        1) The uniform packing particle size ensures high column efficiency
        2) Low column pressure and high resolution
        3) High temperature resistance 85 ℃
        4) High chemical stability and long column life
        5)There are more filler sizes to choose from: 5um, 6um, 8um, 9um, 10um, which can meet the separation requirements of various standards and different samples

        Related technical parameters of target carbohydrate analysis chromatographic column packing

        Column model

        Target-Hydrogen

        Target-Calcium

        Target-Pb

        Target-K

        Target-Na

        Filler matrix

        PS/DVB

        PS/DVB

        PS/DVB

        PS/DVB

        PS/DVB

        Particle size (μm)

        5, 6, 8, 9, 10

        5, 6, 8, 9, 10

        5, 6, 8, 9, 10

        5, 6, 8, 9, 10

        5, 6, 8, 9, 10

        Crosslinking degree

        5%, 8%10%

        5%, 8%10%

        5%, 8%10%

        5%, 8%10%

        5%, 8%10%

        Filler ion type

        H+

        Ca2+

        Pb2+

        K+

        Na+

        ph stability

        1–3

        5–9

        5–9

        5–9

        5–9

        Maximum withstand voltage

        10% crosslinking degree

        10 μm

        1,500 psi

        1,500 psi

        1,500 psi

        1,500 psi

        1,500 psi

         

         

        5 μm

        1,000 psi

        1,000 psi

        1,000 psi

        1,000 psi

        1,000 psi

         

        8% crosslinking degree

        10 μm

        1,000 psi

        1,000 psi

        1,000 psi

        1,000 psi

        1,000 psi

         

         

        5 μm

        800 psi

        800 psi

        800 psi

        800 psi

        800 psi

         

        5% crosslinking degree

        10 μm

        600 psi

        600 psi

        600 psi

        600 psi

        600 psi

         

         

        5 μm

        400 psi

        400 psi

        400 psi

        400 psi

        400 psi

        mobile phase

        PH 2.5 acidic aqueous solution(H2SO4H3PO4TFA…)

        Ultra pure water

        Ultra pure water

        Ultra pure water

        Ultra pure water

        Recommended flow rate(mL/min)

        7.8x300 mm column

        0.4-0.8

        0.4-0.8

        0.4-0.8

        0.4-0.8

        0.4-0.8

         

        4.6x300 mm column

        0.1-0.3

        0.1-0.3

        0.1-0.3

        0.1-0.3

        0.1-0.3

        Temperature tolerance(℃)

        85

        85

        85

        85

        85


        * it is suggested that the chromatographic column with 5 μ m packing particle size, 5% cross-linking degree and 7.8 × 300 mm specification should be selected, and the flow rate range should be 0.1-0.2 ml / min.

        The ion type of target carbohydrate series analytical column was selected according to the sample type
        Ion Sample application
        H Fermentation products, fruit juices containing organic acids, alcohols and sugars
        Ca It is especially suitable for the separation of monosaccharide, disaccharide, trimeric sugar, tetrasaccharide and sugar alcohol
        Pb Pentose and hexose in wood products
        Dairy products containing sucrose, lactose, etc
        K Sucrose, honey, corn syrup, beet sugar and other plant products containing sugars (containing zwitterionic ions such as betaine and trimethylammonium chloride)
        Na Low sugar, especially in the presence of heavy inorganic sodium ions, such as honey.

        High stability
        Target carbohydrate series analytical chromatographic column adopts excellent filler manufacturing technology and advanced filling technology, which ensures the high stability of the packing. Even at high temperature, target carbohydrates can be stable in pure water and other water buffer systems. As shown in the figure below, the retention time of acid is only changed by 0.5% after continuous flushing 578 times of column volume at 55 ℃ for 6 days.


        Column: 4.6x 250 mm
        Mobile phase: 5mMH2SO4
        Flow rate: 0.30 ml / min
        Temperature: 55°C
        Injection volume: 20μL
        Detection: Ri
        Sample:
        1, Citric acid (50 mM); 2, Aceticacid (100 mM)

        Excellent inter assay reproducibility
        The high inter batch reproducibility of target carbohydrate analytical column is due to the controllable surface chemical modification technology and strict monitoring of polymer resin production process. As shown in the figure below, the retention time error between different batches is within 0.25%.


        Column: 7.8x300mm
        Mobile phase: 2.5mMH2SO4
        Flow rate:0.6ML/ min
        Temperature: 55°C
        Injection volume: 5
        μL
        Detection: Ri
        Sample:Mixture of Citric acid, Succunicacid, Formic acid, Acetic acid  

        Some application cases

        Glucose fructose injection


        Chromatographic : Target calcium 8% 9 μ m 7.8 * 300 mm
        Mobile phase: ultrapure water
        Flow rate: 0.6 ml / min
        Temperature: 85 ° C
        Detector: 1. Rid; 2.UV@200nim;3. UV@284NM
        Injection volume: 20 μ L
        Sample: glucose fructose injection
        Peak:
        1. Glucose
        2. Fructose
        3.5-hydroxymethylfurfural


        Organic acids (maleic acid, malic acid and fumaric acid)

        Chromatographic : Target hydrogen 8% 9um 7.8 * 300 mm
        Mobile phase: 5mMH2SO4
        Flow rate: 0.6 ML / min
        Temperature: 40 °C
        Detector: UV@210nm
        Injection volume: 20μL
        Sample (water as solvent matrix)
        1.5μg / ml maleic acid
        2.1mg/ml malic acid
        10μg / ml fumaric acid

        Mixing of organic acids and alcohols


        chromatographic :Target-Hydrogen 8% 9um 7.8*300mm
        Mobile phase: 2.5mMH2SO4
        Flow rate: 0.6 mL/min
        Temperature: 55%C
        Detector: RID
        Injection volume: 10 μL
        Sample peak:
        1. Maltotriose 2. Maltose
        3. Glucose 4. Succinate
        5. Lacticacid6. Glycerol
        7. Acetic acid 8. Propylene glycol
        9. Methanol10. Ethanol
        11. Butanol



        Ordering Information
        model Ion type Particle size Specifications Part No
        Target-Calcium Ca2+ 5um 7.8*300mm 301811-78030 
        Target-Calcium Ca2+ 8um 7.8*300mm  301818-78030
        Target-Calcium Ca2+ 6um 7.8*300mm  301816-78030
        Target-Calcium Ca2+ 9um 7.8*300mm 301819-78030
        Target-Calcium Ca2+ 10um 7.8*300mm 301809-78030
        Target-Hydrogen H+ 5um 7.8*300mm 301800-78030
        Target-Hydrogen H+ 8um 7.8*300mm 301828-78030
        Target-Hydrogen H+ 6um 7.8*300mm 301826-78030
        Target-Hydrogen H+ 9um 7.8*300mm 301812-78030
        Target-Hydrogen H+ 10um 7.8*300mm 301820-78030
        Target-Na Na+ 5um 7.8*300mm 301825-78030
        Target-Na Na+ 8um 7.8*300mm 301608-78030
        Target-Na Na+ 6um 7.8*300mm 301836-78030
        Target-Na Na+ 9um 7.8*300mm 301829-78030
        Target-Na Na+ 10um 7.8*300mm 301830-78030
        Target-Pb Pb2+ 5um 7.8*300mm 301700-78030
        Target-Pb Pb2+ 8um 7.8*300mm 301708-78030
        Target-Pb Pb2+ 6um 7.8*300mm 301846-78030
        Target-Pb Pb2+ 9um 7.8*300mm 301719-78030
        Target-Pb Pb2+ 10um 7.8*300mm 301710-78030

        * tips: if you need more column with other crosslinking degree, particle size and specification, please consult your sales consultant.
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