How to use and maintain chromatographic column?

Maintain chromatographic column
The protective column is a short column with packing similar to that of the separation column, which is connected in front of the separation column or replaces the flow path filter. The function of the protective column is to collect and block the chemical waste at the opening of the separation column. If the garbage enters the separation column directly, it will gradually accumulate on the column head, and eventually reduce the column efficiency, If plasma samples are analyzed, new cores should be replaced after 50 samples are analyzed
The main causes of high pressure shock are: slow rotation of sample valve in injector, too fast pump start-up, column cutting and other operations. The pressure changes little when using manual injection valve, and the automatic injection valve switches slowly, May cause pressure shock
As we know, the chromatographic column is mainly selected according to the molecular weight of the component to be measured, and the mobile phase conditions, including pH value, ionic strength and solvent polarity, etc, High purity silica gel particles (such as Xterra column of waters company) developed by advanced hybrid particle technology combine the advantages of silica gel and porous polymer filler, and make organic silane unit replace a considerable part of silicon hydroxyl groups occupying the internal and surface positions of particles, The new packing has the advantages of high resolution, strong stability and wide pH range (1-12). Its performance has broken through the limit of traditional high performance liquid chromatography column. Therefore, it is necessary to select the appropriate separation column according to the analysis object and the conditions of mobile phase, and consult the technical personnel of the company if you are confused
Wash the separation column regularly. At the end of each analysis, after washing the buffer salt, the column should be washed with strong solvent. For example, chromatographic pure methanol and acetonitrile can be used to wash the reversed-phase C18 column, so as to wash away the strong retention components adsorbed on the column. If methanol / water is used as the mobile phase, the column should also be washed in this way, which can restore the column efficiency to a certain extent
For more related technologies, please consult MS technologies technicians