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        Pharmacopoeia column

        Target -Ephedrine

               The special column for ephedra was filled with polar ether and phenyl bonded silica gel, and the packing was uniform spherical particle with carbon loading of 11.0%. In the separation process under Pharmacopoeia chromatographic conditions, the peak symmetry of ephedrine and pseudoephedrine is good, and the number of theoretical plates is more than 10000, which is far higher than the requirement of "the number of theoretical plates should not be less than 3000 calculated according to the peak of ephedrine hydrochloride" stipulated in Pharmacopoeia.

        Chromatographic conditions of ephedrine hydrochloride
        Chromatographic column: target - ephedrine (5 μ m, 4.6 × 250 mm)
        Mobile phase: 0.092% phosphoric acid solution (containing 0.04% triethylamine and 0.02% di-n-butylamine-methanol (98.5:1.5) (V / V)
        Flow rate: 1.0ml/min
        Column temperature: RT
        Detection: UV 210 nmSample
        : 1. Ephedrine hydrochloride (0.03768 mg / ml)
         2. Pseudoephedrine hydrochloride (0.04648 mg / ml)Injection volume: 10 μ l pressure: 2100 PSI

        name

        retention time

        %Area

        Resolution

        Tailing factor

        Number of trays

        unknown

        3.75

        4.04

         

        1.79

        3837.7

        ephedrine

        9.39

        43.16

        18.52

        0.93

        10649.1

        Pseudoephedrine

        11.56

        52.79

        5.18

        1.29

        10144.2

         

        Part No

        Product description

        Sample test

        301803-4625

        Target -Ephedrine 5um  250*4.6mm

        Special column for Ephedra


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