Tel:06021-58830-11

        Contact us

        06021-58830-11
        Mod:06021-58830-11
        Fax:06021-58830-0
        Add:Zeppelinstr. 5, 63741 Aschaffenburg, Germany

        SPE

        Nav:Home > products > SPE >

        MS-CLEAN VDR

        The pretreatment products of MS clean VDR, combined with hydrophobic action and acid-base theory, can selectively capture phospholipid interferences that lead to ion inhibition without affecting the loss of target substances, provide excellent purification effect for fat containing samples, improve the sensitivity of the instrument to detect analytes, and reduce the maintenance cost of the instrument to a large extent
        Product features
        ● effectively remove phospholipid and reduce ion inhibition effect
        ● one time treatment of multiple animal residues, saving time
        ●No need for activation, rinsing, easy operation and stable recovery
        ●More pure sample, cleaner mass spectrum source, less data error

        Prat No

         Specifications

         packing 

         MSV330

         MS- -clean VDR, 300 mg/3 mL

         50branch

         202002-2110

         Target-core C18 (2.1mmx100 mm, 2.7 um)

         1root

         MSi2500

         MS-Mix Multifunctional mixer

         1platform 

        Comparison of purification effect of GC MS / MS matrix


        Analysis of 19 veterinary drug residues in pork by UPLC / MS / MS
        1 sample extraction
        1) Weigh 2.0g pork into a 50ml centrifuge tube, add 2.0ml of 0.1M nazedta mcllvaine buffer solution, mix with vortex for 1min, extract with ultrasonic wave in ice bath for 10min, centrifuge for 5min at 4 ° C 9000r / min, and transfer it to another 15ml centrifuge tube; 2) add 7.0ml of 2% ethyl formate into a 50ml centrifuge tube, vortex for 5min, and centrifuge for 5min at 4 ° C 9000r / min, Combine the supernatant twice, and use 2% ethyl formate to fix the volume to 10m L; 3) centrifugate the supernatant after the volume is fixed at 4 ° C 9000r / min for 5min, to be purified!
        2 sample purification
        1) Sample loading: take 2.5ml of the solution to be purified to pass through the column, and then put it into prison under the action of gravity, so as to make the effluent deaf; 2) elute: add 625 "80% acetonitrile water for secondary elution, collect the effluent, apply Zhendeng e empty liquid, and combine the effluent twice; 3) add 0.5ml of the sample eluent and 0.3ml of HQ into the sample bottle, vortex and mix well, and pass through 0.22 μ m organic filter membrane for on-board test.
        3 preparation of standard Koji
        The standard koji was prepared with empty matrix and quantified by internal standard method. The concentration range of standard koji was 0.5-100ppb
        4. Instrument test conditions
        (for reference only) chromatographic condition instrument: UPLC / MS / MS (Thermo Fisher TQS Endura) chromatographic column: target core C18 (2.1mmx50 mm, 1.8 PM) column temperature: 30 ° C
        Injection volume: 8 pl
        Flow rate: 0.4ml/min
        Mobile phase: A: water (0.1% formic acid) B: methanol (0.1% formic acid)
        Table 1 gradient elution

        Mass spectrometry conditions
        Ion source: HESI sheath gas pressure: 40 ARB ion transfer tube: 380 ° C
        Electrospray voltage: 3500 V auxiliary gas pressure: 2 ARB auxiliary gas temperature: 420 degrees C
        matter
        1. In order to prevent the loss of tetracycline compounds caused by chelation, aqueous extraction was carried out with 0.1M Na2EDTA buffer solution.
        2. In order to improve the phase separation from solid residues, it is recommended to use low temperature centrifugation (4 ° C) to reduce the impact of temperature on some targets (such as tetracyclines).
        3. High speed centrifugation (9000r / min) is recommended to further ensure the effective removal of protein and some solid residues
        4. It is suggested to add internal standard and prepare standard curve with blank matrix, which is helpful to correct the recovery of target analyte
        experimental result
        Table 2. Experimental results of recovery of 19 kinds of animal residues in pork of different brands with standard addition concentration of 16ng / ml, n = 3

        Before:MS-Clean? HLB-MCX-MAX-WCX-WAX

        Next:no

        Back
        Contact Feedback
        Tel:06021-58830-11
        Sitemap( Google
        Copyright:MS Technologies Tel:06021-58830-0  Fax:06021-58830-11    Add:Zeppelinstr. 5, 63741 Aschaffenburg, Germany

        MS Technologies

        主站蜘蛛池模板: 欧美性大战XXXXX久久久√| 色综合天天综合网国产成人| 小说区乱图片区| 亚州**色毛片免费观看| 男人j进女人p免费视频| 国产偷亚洲偷欧美偷精品| 5555在线播放免费播放| 客厅餐桌椅子上波多野结衣| 久久国产精品久久国产精品| 24小时免费看片| 特级黄色毛片视频| 国产亚洲欧美日韩在线看片| 2019亚洲午夜无码天堂| 女人张开腿日出白浆视频| 久久久久亚洲av综合波多野结衣 | 好男人好视频手机在线| 久久人人爽人人爽人人av东京热| 欧美日韩中文国产一区| 免费超爽大片黄| 被猛男cao男男粗大视频| 国产精品一卡二卡三卡| 99热在线观看| 性一交一乱一伦一| 久久亚洲国产精品五月天婷| 欧美一级特黄乱妇高清视频| 亚洲视频在线观看网站| 精品视频一区二区三区四区| 国产免费看插插插视频| www.五月婷| 国产高跟踩踏vk| jizzyou中国少妇| 成人亚洲成人影院| 久久久久亚洲精品男人的天堂| 桃花直播下载免费观看| 亚洲欧美色一区二区三区| 精品国产免费一区二区三区香蕉 | 中国一级全黄的免费观看| 日本高清视频色wwwwww色| 亚洲专区一路线二| 欧美精品v国产精品v日韩精品| 偷自拍亚洲视频在线观看|